OSM Facility | IST

Leica SP5

Laser scanning confocal microscope for optically sectioned fluorescence imaging.

Laser scanning confocal microscope for optically sectioned fluorescence imaging.

Leica · TCS SP5 confocal microscope

What it does

A focused laser scans the sample and a pinhole rejects light from outside the focal plane, so each image is a clean optical section rather than a blur of everything above and below. Stacking sections reconstructs the sample in three dimensions, with several fluorescent labels separated by wavelength.

Key capabilities

  • Optically sectioned fluorescence imaging
  • Three-dimensional image stacks
  • Multi-channel spectral detection
  • Time-lapse acquisition

Applications

Life sciences · Biotechnology · Materials · Nanoscience

Sample types

Fixed and live cells, tissue sections, labelled biological samples and fluorescent materials.

Technical specifications

ParameterValue
Laser linesTo be confirmed
ObjectivesTo be confirmed
Detection channelsTo be confirmed
Scanning modesTo be confirmed
Confirmed values are pending from the facility. Nothing here is estimated.